Sakura OCT 4583 cryo tissue embedding compound

Introduction

Sakura OCT 4583 cryo embedding compound is a water soluble blend of glycols and resins that provides a stable specimen matrix for cryostat tissue sectioning at temperatures of -10C and below. The compound has the form of a gel at room temperature, but is solidified at -10°C and below. It can be easily removed with water and/or alcohol at room temperatures. Although developed and optimized for cryo ultra-microtomes, it is also an excellent mounting medium for SEM cryo stages and cold stages. The OCT compound is a non-conductive material by nature, but conductivity can be introduced by mixing it with carbon powder.

Features of the Sakura OCT 4583 embedding compound are:

• Water soluble blend of glycols and resins that provides a stable embedding matrix
• Superior section quality at cryostat temperatures from -10°C to -40°C
• Highly soluble in water, alcohol and formalin
• Will not dull cryostat blades
• Does not leave residue on slides during staining procedures, eliminating undesirable background staining
• Tissue, mounted in OCT compound can be frozen between -10°C and -80°C
• Can be used as mounting medium on SEM cryo stages an SEM cold stages